On June 2th, 2014, Nature Communications published the academic paper from Wuhan National Laboratory for Optoelectronics (WNLO) Prof. Shaoqun Zeng’s group and Prof. Hui Gong’s group from the Biomedical Photonics Division, WNLO cooperatively completed one significant paper titled “Chemical Reactivation of Quenched Fluorescent Protein Molecules Enables Resin-embedded Fluorescence Microimaging”.
The method in the paper has provided new access to high-resolution fluorescence microimaging of macroscopic tissue blocks, solving the problem that the well-established traditional resin embedding technique to prepare biological specimens for microscopic imaging is not compatible with the modern green-ﬂuorescent protein (GFP) ﬂuorescent-labeling technique because it signiﬁcantly quenches the ﬂuorescence of GFP and its variants.
The paper has shown most of the quenched GFP molecules are structurally preserved and not denatured after routine embedding in resin, and can be chemically reactivated to a ﬂuorescent state by alkaline buffer during imaging, demonstrating fluorescence microimaging of resin-embedded EGFP/EYFP-labeled tissue block without noticeable loss of labeled structures. The work has provided a turning point for the imaging of ﬂuorescent protein-labeled specimens after resin embedding.
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